The effects of inhibitors of DNA synthesis can be studied in an in vitro replication system...

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Biology

The effects of inhibitors of DNA synthesis can be studied in anin vitro replication system containing E. coli enzymes. In thissystem replication of M13 DNA is sensitive to the drug rifampicin,which inhibits hostâ€“cell RNA polymerase, but replication of the E.coli cellular DNA is not.
a) What do these results suggest about the priming enzymesinvolved in replication of M13 DNA and E. coli DNA?
b) What would be the effect of a mutation disrupting the5Â´--> 3Â´ exonuclease activity of DNA pol I on the replication ofE. coli DNA?

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ANSWER A From the above mentioned data it is clear that the Ecoli is the host and the Bacteriophage M13 is the cell which is infecting the host This uses the host genetic machinery to replicate and transcribe its genome The M13 phage has a single stranded DNA and is usually replicated in the presence of See Answer

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