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  3. the effect of an inhibitor on an enzyme was tested

The effect of an inhibitor on an enzyme was tested and the experiment gave the results...

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Chemistry

  1. The effect of an inhibitor on an enzyme was tested and theexperiment gave the results below. Plot on Excel the data using adouble-reciprocal plot (Lineweaver-Burke), determine Kmand Vmax of the no inhibitor and each type of inhibitorand the type of inhibition that is occurring.

[S] µM      V(µmol/min) V (µmol/min) V (µmol/min)

                 with 0.0 nM    with 25 nM    with 50 nM

                 Inhibitor         Inhibitor         Inhibitor

______     ___________ ___________ ___________

                   0.4         0.22               0.21                0.20

                   0.67       0.29               0.26                0.24

                   1.00       0.32               0.30                0.28

                   2.00       0.40               0.36                0.32

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