The E. coli LacZ will be used as a reporter gene and
the expression of the...
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The E. coli LacZ will be used as a reporter gene andthe expression of the LacZ gene product will be detected by theenzymatic cleavage of the compound X-gal producing blue stainingwithin the embryo. Why was an E. coli LacZ gene used as areporter gene?
Why is there a Drosophila weak basal promoter locatedon the P[lacZ] enhancer-trap element?
The E. coli LacZ will be used as a reporter gene andthe expression of the LacZ gene product will be detected by theenzymatic cleavage of the compound X-gal producing blue stainingwithin the embryo. If blue staining is observed, what does thatindicate about the P[lacZ] enhancer-trap element withinthe Drosophila embryo?
What classes of genes will be studied in this experiment?
When you examine your prepared slides you observe that someembryos do not show a staining pattern, give a technical reason forthis observation. Give a biological reason for thisobservation.
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Answer The lacZ was used as a reporter gene because this allows visual selection of transformants which can be easily analysed to identify the recombinants ie the cells with our gene of interest Also this
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