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The data to the below were collected for the bovine carbonic anhydrase-catalyzed reaction described by CO2 (aq)...

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Chemistry

The data to the below were collected for the bovine carbonicanhydrase-catalyzed reaction described by

CO2 (aq) + H2O (l) -->H+ (aq) + HCO3- (aq)

[CO2] (mmol * L^-1)

R (ݵmol * L^-1 * s^-1)

1.25

28.74

2.50

48.61

5.00

80.28

20.00

155.59

Use these values to determine the value of KM, theMichaelis-Menten constant. Answer the four parts below.

a) If you were to plot this data to graphically determine Rmaxand KM using a Lineweaver-Burk plot, what would you plot for thefollowing data? If you would plot the original data, enter theoriginal value in the corresponding blank.

[CO2] (mmol * L^-1) graph as R (ݵmol * L^-1 * s^-1) graphas

1.25--> ________ 28.74 --> ________

20.00--> ________ 155.59 --> ________

b) Calculate the slope using the data points from part (a). Thendetermine the y-intercept using the second point.

Slope= _______ s y-intercept= ___________ L * s * mmol^-1

c) Use the y-intercept to calculate Rmax.

Rmax=___________ mmol * L^-1 * s^-1

d) Use Rmax and the slope to calculate Km.

Km=___________ mmol * L^-1

Answer & Explanation Solved by verified expert
3.7 Ratings (672 Votes)
Ans a Note thatthe question seeks rate of reaction and other variables in terms ofmmol whereas the question gives the rate of    See Answer
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