Logo
Home Study Material Contact Search
AI Chat FREE
  1. sciences
  2. biology
  3. the bacterial one-hybrid assay holds e. coli cells

The bacterial one-hybrid assay holds E. coli cells hostage until our protein of interest can active...

90.2K

Verified Solution

Question

Biology

The bacterial one-hybrid assay holds E. coli cellshostage until our protein of interest can active transcription andtranslation of enzymes from the HIS3 and URA3 genes. These enzymesare needed to produce Histidine and Uridine, which are both neededfor cells to grow and divide. Make an estimate, based on enzymekinetics and any other information you may need, of how many HIS3and URA3 enzymes will an E. coli cell need to grow anddivide at a normal rate. How many are needed when we add 10 mM3-amino-1,2,4-triazole (3AT) to the growth medium as a competitiveinhibitor of HIS3?

Answer & Explanation Solved by verified expert
3.7 Ratings (476 Votes)
Transcription factor has two domains One is for DNA binding and another for VP16 activation One protein is fused to the DNAbinding domain of LexA repressor to form bait Another protein is fused to the VP16 activation    See Answer
Get Answers to Unlimited Questions

Join us to gain access to millions of questions and expert answers. Enjoy exclusive benefits tailored just for you!

Membership Benefits:
  • Unlimited Question Access with detailed Answers
  • Zin AI - 3 Million Words
  • 10 Dall-E 3 Images
  • 20 Plot Generations
  • Conversation with Dialogue Memory
  • No Ads, Ever!
  • Access to Our Best AI Platform: Flex AI - Your personal assistant for all your inquiries!
Become a Member

Other questions asked by students

Statistics
1.2K views

A simple random sample of size n is drawn from a population that is normally distributed....

Biology
1.2K views

ime Left 0 23 02 2 5 Shafiqa Hamzi Attempt 1 Cytoplasm Mitochondria Nucleus Golgi...

Physics
1.2K views

3 An electric dipole of dipole moment 10 9 C is directed towards negative Y...

Accounting
1.2K views

Explain two challenges in implementing MFRS13 Fair Value Measurement standard. Then, suggest ways to improve...

Accounting
1.2K views

Reed Corp. has set the following standard direct materials and direct labor costs per unit...

Accounting
1.2K views

Current Attempt in Progress Green Landscaping Inc. is preparing its budget for the first quarter...

Accounting
1.2K views

please make answer neat & clearly typed answer is better How to prepare...

Accounting
1.2K views

Which of the following risks may not be accounted for using hedge accounting? Multiple Choice...

Accounting
1.2K views

Requirement 2. Journalize any required entries from the bank reconciliation. (Record debits first, then credits....

Accounting
1.2K views

Consider Corp X is introducing a product on a trial basis and the cost is...

Accounting
1.2K views

What is the difference between a nonprofit merger and a nonprofit acquisition?

Accounting
1.2K views

Please answer clearly and in the same format. Thank you!!!! Required information [The following...