The 2006 research paper by Paredez et al (full reference details below) provided direct evidence linking...

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Biology

The 2006 research paper by Paredez et al (full reference detailsbelow) provided direct evidence linking a role for microtubules inregulating cellulose microfibril deposition in the plant cell wall.Describe the major experimental findings reported in this paper andthe significance of these findings to resolve long standing modelsthat attempted to explain a role for cortical microtubules incontrolling the direction of cellulose microfibril deposition. Inexplaining the experimental strategy used in this research, whatwas the rationale for using a CesA6 mutant line as part of theirexperimental approach?
Reference: Paredez, Somerville and Ehrhardt (2006) Visualizationof cellulose synthase demonstrates functional association withmicrotubules. Science 312: 1491-1495.

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Major findings The process of cellulose deposition in living cells was demonstrated in a transgenic Arabidopsis having a fusion of a functional yellow fluorescent protein and cellulose synthase CESA Confocal microscopy shows movement and alignment of CESA complexes in the plasma membrane The CESA complexes are arranged at constant rates in linear tracks This observation is similar to that of cortical microtubules The movement of microtubules is bidirectional Cellulose synthase complexes containing CESA6 See Answer

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