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Temperature-sensitive mutations (ts) in E. coli are a type of conditional lethal mutation. A conditional lethal...

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Biology

  1. Temperature-sensitive mutations (ts) in E. coli are atype of conditional lethal mutation. A conditional lethal meansthat the mutation is only deadly under certain growth conditions,in this case when the temperature is increased to the restrictivetemperature. The proteins are fully functional at the lowertemperature (permissive). This type of mutation has beenindispensable for studying the essential process of DNAreplication. Mutants in proteins that are necessary for DNAreplication can be classified into either quick-stop or slow-stop.Quick-stop mutants arrest DNA replication immediately, butslow-stop mutants halt after several minutes. Fill in the chartbelow to indicate which mutations will be slow and which quick whenshifted to the higher, restrictive temperature. Assume that theE. coli have already started replication. Explain youranswers. (6pts total: 1.5ptseach)

Mutated Protein

Quick or slow?

Explanation

DNA gyrase

DnaA

DNA helicase

DNA ligase

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