Talking about Chromatography using kool aid, 70% isopropanol,25% isopropanol and 5% isopropanol what is the answer to thequestions?

1. Describe h0w you think the separation is occurring. What isthe mechanism of the separation?

2. Why do you think the consistency of the solution added to thecolumn was changed throughout the separation? This process iscalled gradient elution.

3. All chromatography systems consist of a mobilephase and a stationary phase. Define thesystem: what is/are the stationary phase(s), mobile phase(s),sample(s), analyte(s)?

4. Rank each colored component from least polar to most polar.Justify your ranking based on the order of elution.

Use chemfinder to find the chemical structures of the dyes. Drawthe structures and justify your ranking based on theirstructures.

5. Many analytical procedures require a separation(chromatography) step prior to the analysis. Why?

6. This lab is a “qualitative” lab. It could easily be modifiedto be a “quantitative” lab. Briefly describe how you could makethis a quantitative lab, i.e., how could you determine theconcentration of the dyes in the Kool-Aid sample?

7. Compounds need not be colored to be analyzed usingchromatography. How could colorless compounds be detected in achromatography system?