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Suppose that you are given the task of determining the SCN- content of a blood sample...

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Chemistry

Suppose that you are given the task of determining theSCN- content of a blood sample by forming theFe(SCN)2+ complex and measuring the absorbance. Youbegin by taking a 10.00-mL volume of the blood sample, centrifugingit, transferring 0.50 mL of the clear serum to a flask, and thenadding 15.00 mL of 0.20M Fe3+. The %T of this solution,measured at 460 nm in a 1.0-cm diameter cuvet, is 75%. Acalibration curve, prepared from Fe(SCN)2+ standards,indicates that the Fe(SCN)2+ concentration of thediluted sample solution is 0.033 mM. Calculate the SCN-content (in mM) of the original blood sample.

(Atomic weight of Fe = 55.85 g/m; formula weight ofSCN- = 58.08 g/m)

A) 0.033mM                                                                              

B) 0.33mM                                             

C)0.40 mM

D) 0.82mM         

E) 1.02 mM

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