Some scientists believe that fructose is particularly bad forhuman health because it promote fat synthesis, contributing todevelopment of type II diabetes and fatty liver disease. You are ascientist working for a lab attempting to develop a drug to helpwith such diseases called fructono. The idea is that fructono couldbe added to the diet to reduce fructose absorption; even thoughfructono would be absorbed, it would not be metabolized, and wouldbe excreted by the kidney. The hypothesis is that fructono binds tothe fructose transporter, competitively displacing fructose. Thiscannot be easily studied in humans, so you are investigating thishypothesis with a mouse small intestine, which is known totransport fructose using a transporter that is quite similar inamino acid sequence and structure to the human protein. For yourresearch, you set up a perfused intestine prep. You cut out about 1cm of small intestine, and put it in a dish containing saline. Youinsert a tube into the intestinal section, and use a pump to pushany fluid you want through the lumen of the intestine. You haveradio-labeled fructono, so you can measure its appearance in thesaline outside the intestine. How can you measure the rate oftransport of fructono? 1 pt Describe experiments that would testwhether the absorption of fructono is protein-mediated vs. passive(just leaking through cracks, or diffusing through membranes). Whatresults will you get if the transport is protein-mediated vs.passive? 2 pts. Describe experiments that would test whether theabsorption of fructono is active, and the predicted results iftransport is active or not. 2 pts. Describe experiments that willtest whether the active transport of fructono requires luminal Na+,and the predicted result if transport is Na+-dependent or not. 1pt. Describe experiments that will test whether fructono can reducethe transport of fructose in a dose-dependent manner. 2 pts Whatside-effects might fructono have? 2 pts