Showing each step: Use the molecular weight of Tyrosinase, 128,000, to determine how many micromoles of Tyrosinase...

4.1 Ratings (777 Votes)

Given concentration of Tyrosinase is 15 mg/mL.Â Â

That means 1 mL of the solution contains 15 mg of Tyrosinase

Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â 5 mL of the solution contains 15x5 = 75 mg of Tyrosinase

Given molecular weight of Tyrosinase =128,000 g/mol

We know that number of moles , n = mass/molar mass

Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â = 75 mg / (128000 (g/mol))

Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â = 75x10^-3 g / (128000 (g/mol))

Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â = 5.47x10^-8 mol

Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â = 5.47x10^-2x10^-6 mol

Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â = 5.47x10^-2 micromol

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