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Show all calculations in a neat and organized manner. Be sure that I understand your logic....

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Biology

Show all calculations in a neat and organized manner. Be surethat I understand your logic. For your experiment you must treat 1X 109 cells with 5 nM cycloheximide (MW=281.35). Cycloheximide isboth toxic and expensive. You have counted the cells with ahemocytometer and found the following numbers when counting 5 “4x4”sections (each 4x4 is 0.004 µl) of a 1:10 dilution of you chlamystock: 90, 103, 123, 99, 107.

1) What is the concentration of the initial Chlamy culture incell/ml? _______________________

2) How many mls of cells of the culture must you use for theexperiment? _____________________

3) Describe how you will treat the cells with cyclohexamide.Clearly show all calculations.

Answer & Explanation Solved by verified expert
3.6 Ratings (636 Votes)
1 volume of each 44section 0004 uLvolume of 5 44section 50004 002 uL1 uL 103 mL002 uL 002 103 2 105 mLaverage number of cells counted 90103123991071044number of cells in 2 105 mL 1044number of cells in 1 mL 1044 2 105 522 106 cellsnumber of cells in 1 mL of the original culture number of    See Answer
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