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sanger sequencing, and how base identity (A,T,C,G) is detected using fluorescence. Can you explain what it...

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Biology

sanger sequencing, and how base identity (A,T,C,G) isdetected using fluorescence. Can you explain what it means that theDNA is labeled with a fluorescent tag, and where that tag islocated?

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sanger sequencingIt is a method of identifying the nucleotide bases and order ofarrangement of these nucleotide bases on DNAIt is called sanger sequencing as it was first development byFrederick Sanger and colleagues in 1977This is also called as chain termination or dideoxy methodThe principle behind this is utilization of specially designednucleotides2 3 ddNTPSDideoxy nucleotide triphosphates whichacts as terminator molecules stops chain elongation ie they donot form phosphodiester    See Answer
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