RNAi is used to functionally inactivate genes in cells and whole organisms like C. elegans. Describe...

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Biology

RNAi is used to functionally inactivate genes in cells and wholeorganisms like C. elegans. Describe the basics of how you wouldknock down the expression of a gene required for muscle formationin C. elegans and what method could you use to confirm that yourresults were specifically attributed to the RNAi?

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RNA interference RNAi is a commonly used technique for reverse genetic approaches in Caenorhabditis elegans Feeding RNAi is the most convenient and inexpensive method for performing genomewide RNAi screens In Caenorhabditis elegans many of the critical proteins involved in this process have been uncovered through mutational screens focusing on uncoordinated movement and embryonic arrest phenotypes Caenorhabditis elegans is a valuable model organism for the study of muscle due to the similarity of worm body wall muscle to vertebrate muscle along with its semitransparent cuticle that allows for visualization of muscle structures in vivo The basic protein components within a C elegans sarcomere have vertebrate counterparts with only minor differences in protein composition and organization MATERIALS AND METHODS Acquiring C elegans strains Wildtype C elegans strains Preparing E coli OP50 See Answer

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