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Revertant colonies plate 1500 1000 500 O Missense mutations 20 O Frameshift mutations O Nonsense...

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Revertant colonies plate 1500 1000 500 O Missense mutations 20 O Frameshift mutations O Nonsense mutations TA 100 TA 1538 Guanine 40 60 80 100 Aflatoxin B dose ng O All except frameshift mutations Aflatoxin B The graph shows the results of an Ames test to identify the effect of several concentrations of Aflatoxin B using a couple of his Salmonella strains TA 100 and TA 1538 While TA 100 is a strain sensitive to reversion mutations by base substitutions TA 1538 is sensitive to frameshift mutations Based on the results what kind of mutations can Aflatoxin B1 lead to CH The TA 100 and TA 1538 are his Salmonella bacterial strains sensitive to 120 reversion mutations by base substitutions and frameshifts respectively Aflatoxin is a mutagen that binds to G bases and induces apurinic sites in DNA

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