Questions 9 to 13 are in reference to the DNAsequence shown in Question 8.
Here is Question 8.
Question 8:
The top strand of the following segment of DNA serves as thetemplate strand:
3’ TACACCTTGGCGACGACT 5’
5’ ATGTGGAACCGCTGCTGA 3’
We will refer to this segment of DNA as the original (orunmutated) sequence.
Please answer the following questions:
(a) What is the mRNA sequence?
The mRNA sequence is  5'  3'.
**Please enter your sequence in the 5' to 3' direction.Deductions will be made if a sequence is inputted in the wrongdirection.**
(b) Using the mRNA sequence you determined in part (a)of this question, give the sequence of the protein that would betranslated.
The amino acid sequence for this protein isN-terminus  C-terminus.
**Please note**
The N-terminus refers to the beginning of the primary sequencefor a protein, and the C-terminus refers to the end of the primarysequence for a protein.
i.e. input the amino acids in the order that they would betranslated.
If a codon encodes for a stop codon, type STOP.
When inputting your sequence, separate each amino acid with ahyphen (e.g. Ser-Tyr-STOP).
You will need to consult the genetic code to answer thisquestion.
Question 10:
The original (unmutated) DNA sequence (shown above in Question8) has been mutated to the following (this represents the templatestrand):
3’ TACGACCTTGGCGACGACT 5’
We will refer to this sequence as mutation#2.
Please note that for simplicity only the template strand forthis mutated segment of DNA is shown.
Answer the following questions:
(a) What is the complete mRNA sequence for the mutatedsegment mutation #2?
The mutated mRNA sequence is  5'  3'.
**Please enter your sequence in the 5' to 3' direction.Deductions will be made if a sequence is inputted in the wrongdirection.**
(b) Using the mRNA sequence you determined in part (a)of this question, give the sequence of the protein that would betranslated.
The amino acid sequence for this protein isN-terminus  C-terminus.
**Please note**
The N-terminus refers to the beginning of the primary sequencefor a protein, and the C-terminus refers to the end of the primarysequence for a protein.
i.e. input the amino acids in the order that they would betranslated.
If a codon encodes for a stop codon, type STOP.
When inputting your sequence, separate each amino acid with ahyphen (e.g. Ser-Tyr-STOP).
You will need to consult the genetic code to answer thisquestion.
4 points  Â
QUESTION 11
Questions 9 to 13 are in reference to the DNAsequence shown in Question 8.
Question 11:
The original (unmutated) DNA sequence (shown above in Question8) has been mutated to the following (this represents the templatestrand):
3’ TACACCTTAGCGACGACT 5’.
We will refer to this sequence as mutation#3.
Please note that for simplicity only the template strand forthis mutated segment of DNA is shown.
Answer the following questions:
(a) What is the complete mRNA sequence for the mutatedsegment mutation #3?
The mutated mRNA sequence is  5'  3'.
**Please enter your sequence in the 5' to 3' direction.Deductions will be made if a sequence is inputted in the wrongdirection.**
(b) Using the mRNA sequence you determined in part (a)of this question, give the sequence of the protein that would betranslated.
The amino acid sequence for this protein isN-terminus  C-terminus.
**Please note**
The N-terminus refers to the beginning of the primary sequencefor a protein, and the C-terminus refers to the end of the primarysequence for a protein.
i.e. input the amino acids in the order that they would betranslated.
If a codon encodes for a stop codon, type STOP.
When inputting your sequence, separate each amino acid with ahyphen (e.g. Ser-Tyr-STOP).
You will need to consult the genetic code to answer thisquestion.
4 points  Â
QUESTION 12
Questions 9 to 13 are in reference to the DNAsequence shown in Question 8.
Question 12:
(1 mark each)
In reference to the original sequence (shown in Question 8),classify each type of mutation present from Questions 9 to 11.Choose the best option for each.
| mutation #1 | | mutation #2 | | Â Â | mutation #3 |
| | A. | base substitution - silent mutation | | B. | insertion - frameshift mutation | | C. | deletion - frameshift mutation | | D. | base substitution - missense mutation | | E. | base substitution - nonsense mutation |
|
QUESTION 13
Questions 9 to 13 are in reference to the DNAsequence shown in Question 8.
Question 13:
Most mutations have a neutral effect on the phenotype, functionor survival of an organism because they do not elicit anynoticeable change. Whereas other mutations can have a positiveeffect on an organism leading to new versions of proteins that helpan organism adapt to changes in its environment; while othermutations can have a negative effect on the organism and result ina protein that does not function normally or at all.
Answer the following questions based on the responsesyou gave above in Questions 8 to 12.
(a) Based on the protein sequences that were produced as aresult of mutation #1, mutation #2, or mutation #3, describe theeffect, if any, these mutations would likely have on the functionof the protein within the cell. Support your answer.
(b) If these mutations occurred within a germline cell and not asomatic cell, how would the effects of these mutations differ?
