Question 5 (alternate). Gene and protein expression (Total 4 marks) A. You have successfully completed your two-channel...

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Biology

Question 5 (alternate). Gene and protein expression (Total 4marks)
A. You have successfully completed your two-channel microarrayexperiment and have obtained Red and Green intensity values foreach feature/probe on the array.
i. Describe how you would initially transform the data so thatincreases and decreases in gene expression are treated equally. (1mark)
ii. What method would you use to normalise the intensities ofthe two dyes, as well as to eliminate other systematic differencesdue to unequal experimental conditions. (1 mark)
B. You wish to normalise data values between arrays. Providedetails of a method for normalising data between arrays.

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A iThe hybridization data of the labeled cDNA is allowed to bind on the mRNA obtained from the cells of the normal samples and affected disease patient samples The signals of the intensity of hybridization are recorded on the microarray screen The data is normalized and the values are given a scaling of 1 for total strong hybridization and a value of 0 for a very low hybridization levels The samples include See Answer

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