Question 11 [10 marks / 10 minutes] For the mutations in the 5â€™ UTR of the...

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Biology

Question 11 [10 marks / 10 minutes]
For the mutations in the 5â€™ UTR of the trp operon given below,explain the effect on expression of the trp operon relative towildtype (increased, decreased, or unchanged).
Assume medium levels of tryptophan, such that TrpR (repressor) isinactive and attenuation is active.
a) A mutation in region 2 such that it cannot bind with region 3.[4 marks]
b) A mutation in region 4 such that it cannot bind with region 3.[4 marks]
c) Insertion of T-A basepairs immediately after region 3, such thaturacils are transcribed. This insertion does not change thestem-loop formation properties of regions 1, 2, 3 or 4. [2marks]

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The trp operon is a repressible operon which means that the gene expression decreases in presence of the substrate for the operon Here the interaction of the substrate with the operon induces a physical change in the structural organization of the See Answer

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