QUESTION 1
We will be using spectrophotometry to determine theconcentration of iron in solutions. First, you will generate acalibration curve. The ----- variable is -----, and will be graphedon the x axis of the calibration curve. The -------- variable is----------, and will be graphed on the --------- axis.
Word Bank:
-independent
-y
-absorbance
-dependent
-concentration
QUESTION 2
How many mL of a 100 mg/dL Ferrozine solution are needed tocomplex 0.00000048 moles of iron?
HINT: Look at the 1st equation in the lab manual (under theheading Background)
PUT YOUR ANSWER IN DECIMALS (not scientific notation)
QUESTION 3
How many moles of Fe3+ can be reduced by 1.1 mL of 0.527 Mhydroxylamine solution?
Look at the 2nd equation in your lab manual under the headingBackground to see the relationship between Iron andhydroxylamine.
Put your answers in DECIMALS, not scientific notation.
QUESTION 4
The trendline equation calculated from a calibration curve ofabsorbance vs iron concentration in μg dL-1 is y = 0.000812x +0.00709. If a sample analyzed using the same assay gives anabsorbance of 0.099, what is the iron concentration of the unknownsample in μg dL-1?
This is a simple \"equation of a line\" formula.
QUESTION 5
As part of this laboratory, you may have to remove iron from asolution. If you have 50 mL of a solution that contains 239 μgdL-1, how many μg of iron must be removed to reach a concentrationof 150 μg dL-1?
HINT: 1. Convert 50mL to dl;
2. Figure out how much μg of solution you have in that 50mL
3. Figure out how much μg of solution you want in 50 mL
4. Subtract the answers from 2 & 3.
QUESTION 6
As part of this laboratory, you may have to add iron to asolution. If you have 100 mL of a solution that contains 45 μgdL-1, how many grams of iron must be added to reach a concentrationof 150 μg dL-1?
Put your answer in scientific notation
QUESTION 7
The concentration of the iron stock solution for this lab is500 μg dL-1. What volume of this solution (in mL) must you add tothe \"patient\" sample to give your patient 0.00000003 moles ofiron?
