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Question 1 Let’s start with a relatively straightforward one – and make it topical. Briefly explain what qPCR...

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Biology

Question 1

Let’s start with a relatively straightforward one – and make ittopical.

  1. Briefly explain what qPCR is and why it is differentthan traditional PCR. Address what the q stands for and why thetechnique is sometimes called real-time pcr.

Unfortunately – that real-time moniker became a problem becausesome people shortened it to RT-PCR.However, most usage of RT-PCR issomething distinct from qPCR.

  1. Describe that something else RT-PCR.

You can combine qPCR and RT-PCR – producing RT-qPCR. Oneapplication of RT-qPCR is detection of certain viruses – such asCOVID-19.

  1. For this application, describe why RT-qPCR ispreferable to qPCR.
  2. For this application, describe why RT-qPCR ispreferable to RT-PCR.

Answer & Explanation Solved by verified expert
4.4 Ratings (858 Votes)
Answer A The qPCR is a technique in molecular biology that monitors DNA amplification during the PCR process is carried out and not at the end of the PCR completion ie like in conventional PCR Tradition PCR or conventional PCR shows the results after loading the sample on an agarose gel and after the entire cycle is over but in realtime PCR we can monitor the amplification per cycle and we    See Answer
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