Q2 A mutant monkey was identified that showed resistance to a viral disease â€˜Kansahâ€™. The kan...

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Q2 A mutant monkey was identified that showed resistance to aviral disease â€˜Kansahâ€™. The kan gene, responsible for the diseaseresistance, was identified and cloned. A molecular techniquerevealed the presence of kan in human cells. Later theseresearchers isolated RNA from liver, kidney, gut and skin cells.The researchers prepared cDNA using reverse transcriptase and thenperformed real-time PCR to measure the expression of the kan gene.The Ct values for samples from gut, kidney, liver and skin cellswere 20, 25, 23 and 38, respectively. Answer the followingquestions
a. What is real-time PCR? How does it differ from the standardPCR?
b. What do you understand by the term Ct value? Explain using afigure.
c. Which cells have the highest expression of kan? Explain youranswer.
d. If a liver cell makes 1,000 molecules of Kan protein, howmany molecules will you expect from a single kidney cell?
e. If a liver cell makes 1,000 molecules of Kan protein, howmany molecules will you expect from a single gut cell?

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Ansa Real time PCR also known as quantitative RT PCR is amethod that is based on general principle of PCR which is used toamplify and simultaneously quantify a target DNA moleculeRT PCRallowedthe scientist or researchers to actuallyvisualize the increase in the amount of DNA as it is amplifiedthats why it is called as real time PCRReal time PCR system based upon the See Answer

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