Q1. Cell survival curves Suppose you did an experiment to generate a cell survival curve. a) Describe what...

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Biology

Q1. Cell survival curves
Suppose you did an experiment to generate a cell survivalcurve.
a) Describe what steps you would take to do the experiment.
b) What is meant by plating efficiency?
c) In your experiment you irradiate cells using 6 MV photons ona linear accelerator. You collect the data shown in the tablebelow. Plot cell survival as a function of dose and use thelinear-quadratic model to fit the data.
d) Determine the alpha/beta ratio from the curve fit. Is this anearly responding or late responding cell line?
Number of cells plated
Number of colonies counted
Dose (Gy)
100
75
0
400
255
2
400
180
5
1000
150
10
10000
90
20

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3.9 Ratings (613 Votes)

A You start by preparing the cells to either be the controlgroup or be one of the treatment groups You plate some cells forthe control group and then you prepare some moer plates dependingon the number of treatments you want to apply that would be oneplate See Answer

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Number of cells plated	Number of colonies counted	Dose (Gy)
100	75	0
400	255	2
400	180	5
1000	150	10
10000	90	20