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  3. q 1: if the fluorescence signal from a pmt is 0.53

Q 1: If the fluorescence signal from a PMT is 0.531 volts, what will the voltage...

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Chemistry

Q 1: If the fluorescence signalfrom a PMT is 0.531 volts, what will the voltage output be if thefollowing occurs:

a. the lamp intensity doubles
b. the concentration is reduced by 1/2
c. pathlength is doubled
d. the quantum yield drops from 0.50 to 0.10

e.temperature increases

Q 2: Predict how the fluorescence signal changes if thefollowing occurs. Explain your answer.

[1] the sample temperaturedecreases

[2] the excitation light intensity increases by 25%

Answer & Explanation Solved by verified expert
4.2 Ratings (536 Votes)
Solution The fluorescence intensity F is proportional to the amount of light absorbed and the fluorescence quantum yield FkIbc where k is a proportionality constant I is the incident light intensity is    See Answer
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