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Puromycin is a powerful inhibitor of protein synthesis. It is an analog of the 3’ end...

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Biology

Puromycin is a powerful inhibitor of protein synthesis. It is ananalog of the 3’ end of aminoacyl tRNA. When puromycin is added toa cell-free system containing all the necessary machinery forprotein synthesis, incomplete polypeptide chains are released fromribosomes. Each such chain has py y uromycin covalently attached toone end.

a) Explain these results.

b) To which end of the polypeptide chains would you expect thepuromycin to be bound? Explain fully.

c) Would you expect puromycin to bind to the A or P site on theribosome, or to both? Explain fully.

Answer & Explanation Solved by verified expert
4.3 Ratings (891 Votes)
a Puromycin resembles its structure with the nucleoside monophosphate and its structure is somewhat same to the 3 end of tRNA Due to its structure similarity it can easily bind to the A site of the    See Answer
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