Please use this paper to answer both the sub section question indetail please. this paper is avialbale online so thier is noplagirism
http://genesdev.cshlp.org/content/9/7/783.full.pdf
Q. How did the use of the cactSu and cactΔPEST versions ofcactus helped to demonstrate the existence of two types ofdegradation that control the levels of Cactus protein.
