Please I need all answers about questions in TLC LAB.

(Q1) In our lab, what is the mobile phase and what is stationaryphase? Discuss the polarity of each.

(Q2) Do your results suggest that the chemical characteristicsof these pigments might differ according to their color?Explain.

(Q3) Which of your pigment molecules was the most non-polar?Polar?

(Q4) What is the relationship between Rf values and polarity forthis experiment? Is this always true?

(Q5) Why should you not use ink on the coating to mark yourpigment placement?

Here is procedure of lab.

Procedure

(1) On a balance weigh ~0.5 grams of algae powder and ~0.5 gramsof anhydrous magnesium sulfate.

(2) Transfer (1) to a large test tube and combine with 2.0 ml ofacetone. Stopper the test tube and shake vigorously forapproximately one minute. You need to make sure that the solventand solid are well mixed. Allow the mixture to stand for fewminutes.

(3) Use a pipette to carefullytransfer the solvent above the solid (should be green) into a smalltest tube. Cover the tube to avoid evaporation.

(4). Obtain a TLC chamber (a glass jar with a cover) and adddeveloping solvent (a mixture of pet ether, acetone, cyclohexane,ethyl acetate and methanol). The solvent should completely coverthe bottom of the chamber to a depth of approximately 0.5 cm.

(5). For each person, obtain a TLC plate (a silica gel coatedplastic sheet) which has been precut and make a dot with a pencilon the coated side (the matt side) approximately 1.0 cm from thebottom of the strip.

(6). Fill a capillary tube by placing it in the leaf extract.Keep your finger on the end of the tube. Apply the extract to thecenter of the dot on the TLC plate by quickly touching the end ofthe TLC applicator to the plate. Allow to dry. Repeat several timesto make a concentrated dot of extract. Be sure to let dry betweenapplications.   The best ‘dot’ would be a concentratedbut small in size. PRACTICE DOTTING ON A PAPER TOWEL BEFORE USINGTHE TLC PLATE !

(7)Carefully place the TLC plate in the TLC chamber. The TLC plateshould sit on the bottom of the chamber and be in contact with thesolvent (solvent surface must be below the extract dot). Screw thelid on the TLC chamber.

(8). Allow the TLC plate todevelop (separation of pigments). As the solvent moves up the TLCplate you should see the different colored pigmentsseparating.

(9). Remove the TLC plate from the chamber when the solvent fromis approximately 1.0 cm from the top of the TLC plate. With apencil, mark the level of the solvent front (highest level thesolvent moves up the TLC plate) as soon as you remove the stripfrom the chamber. IT WILL FADE QUICKLY.

(10) Using a pencil, gently trace all the pigments. These willfade also. Tape the TLC plate onto your lab notebook. Sketch theTLC plate next to it including the solvent line, trace of pigments.Now add on the descriptions such as color to the sketch. Bespecific.