Please explain why B is the correct answer, I know on the 5'---->3' it goes reverse and...

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Biology

Please explain why B is the correct answer, I know on the5'---->3' it goes reverse
and 3'-->5' it goes forward.......I got lost after this.Please explain. Will give a thumbs up.
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Which of the following sets of primers could you use to amplifythe target DNA sequence below, which is part of the lastprotein-coding exon of the gene involved in cystic fibrosis?
5â€™- ggctaagatctgaattttccgag â€¦ ttgggcaataatgtagcgcctt - 3â€™
3â€™- ccgattctagacttaaaaggctc â€¦ aacccgttattacatcgcggaa â€“ 5â€™
a) 5â€™ GGAAAATTCAGATCTTAG 3â€™;
5â€™ TGGGCAATAATGTAGCGC 3â€™
b) 5â€™ GCTAAGATCTGAATTTTC 3â€™;
3â€™ ACCCGTTATTACATCGCG 5â€™
c) 3â€™ GATTCTAGACTTAAAAGGC 5â€™;
3â€™ ACCCGTTATTACATCGCG 5â€™
d) 5â€™ GCTAAGATCTGAATTTTC 3â€™;
5â€™ TGGGCAATAATGTAGCGC 3â€™.

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3.8 Ratings (641 Votes)

Firstly want to explain that the DNA template is read in 3 to 5 direction and a new strand is synthesized in the 5 to 3 direction RNA polymerase uses template strand DNA strands to make a complementary RNA See Answer

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