Please explain why B is the correct answer, I know on the5'---->3' it goes reverse
and 3'-->5' it goes forward.......I got lost after this.Please explain. Will give a thumbs up.
-----
Which of the following sets of primers could you use to amplifythe target DNA sequence below, which is part of the lastprotein-coding exon of the gene involved in cystic fibrosis?
5’- ggctaagatctgaattttccgag … ttgggcaataatgtagcgcctt - 3’
3’- ccgattctagacttaaaaggctc … aacccgttattacatcgcggaa – 5’
a) 5’ GGAAAATTCAGATCTTAG 3’;
5’ TGGGCAATAATGTAGCGC 3’
b) 5’ GCTAAGATCTGAATTTTC 3’;
3’ ACCCGTTATTACATCGCG 5’
c) 3’ GATTCTAGACTTAAAAGGC 5’;
3’ ACCCGTTATTACATCGCG 5’
d) 5’ GCTAAGATCTGAATTTTC 3’;
5’ TGGGCAATAATGTAGCGC 3’.
