Please be as discriptive as possible. (Break it down dummy style please) Regarding the posted article...

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Biology

Please be as discriptive as possible. (Break it down dummy styleplease) Regarding the posted article â€œHepatitis E vaccine debuts:Success of Chinese biotech partnership raises hopes for preventionof overlooked diseasesâ€ (Nature 10/29/2012): The workers made arecombinant subunit vaccine in E. coli host cells. Hepatitis Evirus is a non-enveloped virus that contains an RNA genome andicosahedral capsid. Describe a stepwise process that could be usedto develop and produce the vaccine. You have purified hepatitis Evirus genomic RNA as starting material. (side question: if itâ€™srecombinant would cloning be involved why or why not?)

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The first thing that is required for making the vaccine is determining the sequence of the Genomic RNA sequence which can act as an antigen or can produce a protein that is virulent that can be antigenic and can elicit the immune response in the host So first step is to determine the portion that can act as an antigen and elicit the immune response after that the whole genetic engineering or recombinant DNA technology comes into picture Now we will have to cut our desired gene using restriction endonucleases and isolate the desired gene See Answer

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