Please answer both questions: 1. A number of different buffers/components are used in the PCR. Explain the...

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Biology

Please answer both questions:
1. A number of different buffers/components are usedin the PCR. Explain the function ofÂ each:
DNA plasmid

Primer pair

dNTPs mixture

Taq polymerase
2. In our PCR reaction the optimum annealingtemperature is 56^oC. Explain the general factors thathelp determine the optimum annealing temperature of a PCRreaction

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1DNA plasmid A target gene inserted into a circular piece of DNA called a plasmid plasmid is introduced into bacteria by a process called transformation and bacteria carrying the plasmid are selected using antibiotics Bacteria with the correct plasmid are used to make more plasmid DNA induced to express the gene and make protein PRIMER PAIR Primers are the strands of DNA or RNA that serve as this ini for the DNA replication process and they are used to demarcate the segment of the DNA template to be amplified In the PCR process two primers are matched to See Answer

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