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PLEASE ANSWER ALL PARTS FULLY! How was liquid hybridization used to compare species relatedness? What is the...

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Biology

PLEASE ANSWER ALL PARTS FULLY!

How was liquid hybridization used to compare speciesrelatedness? What is the purpose/significance of Driver versusTracer DNA? What is Subtractive Hybridization, how/why would youuse this technique?

Compare Maxam-Gilbert and Sanger DNA sequencing methods. Whatare the more descriptive names for these techniques? How are thetechniques similar? different? What reagents are used in eachreaction? How are the results read? Do you get the sequence of themolecule you started with or the complementary molecule? How doesfluorescent cycle sequencing differ from the original SangerMethod?

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4.2 Ratings (823 Votes)
In order to find the genetic similarity between pools of DNA sequences DNA hybridisation is used It works by finding out the genetic distance between two organisms The DNA of one organism is labelled then mixed with the unlabelled DNA to be compared against The mixture is incubated to allow DNA strands to separate and form hybrid doublestranded DNA Hybridized sequences with a high degree of similarity will bind firmly and more energy is required to separate them They get separated when heated at a higher temperature this process is known as DNA melting Now the doublestranded DNA is bound to a column and the mixture is heated in steps At each step the column is    See Answer
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