Plant Physiology
suppose you suspect your gene of interest is expressed during
M -phase, what type of...
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Biology
Plant Physiology
suppose you suspect your gene of interest is expressed duringM -phase, what type of experiment(s) might you design to prove yourhypothesis?
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First we can synchronise and sort cells in G1 S G2 and M phase by using Flow cytometer Here first we stain our cell with propidium iodide PI which binds to DNA of cell More DNA intense peak because of more attachment of PI We can sort cells at small peak G1 no DNA replication S phase a progression of peak because start of DNA replication Doubly intense peak at the boundary of G2M phase because here intensity of PI will become double which
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