Plamitate uniformly labeled with tritium (3H) to a specific radioactivity of 3.0×·10^8 counts per minute per...
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Plamitate uniformly labeled with tritium (3H) to a specificradioactivity of 3.0×·10^8 counts per minute per micromole wasadded to a mitochondrial preparation that oxidized 3H-palmitate toacetyl-CoA. The experiment calls for hydrolysis of the recoveredacetyl-CoA to acetate and determination of the specificradioactivity of the acetate in counts per minute per micromole(cpm/μmol). Assuming that the palmitate is oxidized by theβ-oxidation pathway and that there is no unlabeled fatty acid todilute the tritium, what would be the expected specificradioactivity of the acetate? Show your reasoning andcalculations.
Plamitate uniformly labeled with tritium (3H) to a specificradioactivity of 3.0×·10^8 counts per minute per micromole wasadded to a mitochondrial preparation that oxidized 3H-palmitate toacetyl-CoA. The experiment calls for hydrolysis of the recoveredacetyl-CoA to acetate and determination of the specificradioactivity of the acetate in counts per minute per micromole(cpm/μmol). Assuming that the palmitate is oxidized by theβ-oxidation pathway and that there is no unlabeled fatty acid todilute the tritium, what would be the expected specificradioactivity of the acetate? Show your reasoning andcalculations.
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