PCR can generate over 100 _________________ copies of DNA in hours. ____, _____, _____, and _____ are...

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Biology

  1. PCR can generate over 100 _________________ copies of DNA inhours.
  2. ____, _____, _____, and _____ are the nucleotides needed to______ copies of DNA
  3. Why do we need to add primers?
  4. What can DNA Polymerase withstand that other enzymescannot?
  5. When do your desired fragments start to appear?
  6. On cycle ________________, you have over a billion copies ofDNA.
  1. What is the purpose of the agarose gel?
  2. What makes the DNA move?
  3. DNA migrates to the _________________ end of the gel.
  4. True or False: Long strands of DNA migrate farther than shortstrands of DNA.
  5. Why do we stain the gel?
  6. What is agarose made from?
  7. Why do we add buffer into the gel mix?
  8. What is the gel comb used for?
  9. True or False: buffer keeps the gel from drying out.
  10. Why add the loading buffer to the DNA sample?
  11. Why do we also run a standard?
  12. True or False: the red wire will generate a negativecharge.
  13. True or False: because DNA is negatively charged, it willmigrate to the negative pole.
  14. How do we know that the current is running in the gel box?
  15. Ethidium bromide fits between the ___________ of the DNA ladderand shows up under ___________________ light.
  16. Write down your DNA estimates below.

(largest (1) to smallest (3)) bp= basepair

1 ________________bp

2 ________________bp

3 ________________bp

Answer & Explanation Solved by verified expert
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PCR can generate over 100 billion copies of DNA in hours adenine A thymine T guanine G and cytosine are the nucleotides needed to make copies of DNA purpose of PCR primers is to    See Answer
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