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Part A - Short Answer If tryptophan (trp) codons containing two trp codons in its leader region...

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Biology

Part A - Short Answer

If tryptophan (trp) codons containing two trpcodons in its leader region were mutated to serine (ser)codons, then a high level of expression of the mutated operon wouldbe visible ONLY if trp and ser were absent at the sametime from the media. Explain why this occurs.

Part B - Multiple Choice

Circle the correctrelationship between the trp operon and the repressor.

  • The repressor acts in 'cis'.
  • The repressor protein is responsible for the formation of the\"termination\" stem loop.
  • Binding of the repressor protein leads to attenuation of thetrp operon.
  •   The presence of tryptophan results in increasedexpression of the tryptophan gene
  • When bound to tryptophan, the repressor is able to bind theoperator of the trp operon

Part C - Multiple Choice

Before transcription of the lac operon can commence, RNApolymerase must strongly bind to the promoter.Circle the correct statement asto why this occurs.

  • lacI+ is mutated to lacI-
  • a CAP-cAMP complex binds to the CAP site in the promoter in theabsence of glucose.
  • CAP binds to the CAP site in the promoter in the presence ofglucose.
  • catabolite repression occurs in the presence of glucose.
  • catabolite repression occurs in the absence ofglucose.

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