Outline the roles played by restriction enzymes and vectors in cloning DNA. Words to choose from: bacterial,...

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Biology

Outline the roles played by restriction enzymes and vectors incloning DNA.

Words to choose from: bacterial, eukaryotic,nucleotide, DNA, RNA, protein, palindrome,

nuclease, protease, phage, plasmid.

1. A restriction enzyme is a _________     ____________that recognizes specific _________ sequences in a________molecule, often a __________, and cleaves or nicks themolecule at those sites.

2. A vector is an agent into which a foreign DNA segment isinserted and used to transform host cells. A vector can be createdfrom a virus known as a __________ or a bacterial _________.

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Cloning is used for amplification and insertion of gene of interest in expression vector for getting protein in large in large amount In cloning every component is important from vector gene of interest expression cell line restriction    See Answer
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