One thousand bases of quail mitochondrial DNA were downloaded to Geneious from the GenBank database. PCR...
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Biology
- One thousand bases of quail mitochondrial DNA were downloadedto Geneious from the GenBank database. PCR primers were designed toamplify a conserved region from this sequence. Each primer was 20bases long, and the 5` end of the forward primer (F) and reverseprimer (R) starts from base position 33 and 865, respectively. APCR master mixture for 23 reactions to analyse different birdsamples wasprepared.                           Â
- Fill in the necessary information for the master mix in thefollowing table
No of reactions tomake up
23
Vol of each Reaction(µL)
24 µL +1 µL DNA
Total VolumeRequired
_____µL total
Component
Suppliedas
Final ConcinPCR
µLrequired
1rxn       23
MgCl2
rxn’s
MgCl2
25 mM
2.5 mM
Polymerase buffer
5x
1x
Primer forward(F)
10 µM
1 µM
Primer Reverse(R)
10 µM
1 µM
Taq DNApolymerase
1Units/µL
1 U per rxn
dNTPs
25 mM
1 mM
Template DNA addedlater
-
-
Water
-
-
Total volume
25 µL
- One thousand bases of quail mitochondrial DNA were downloadedto Geneious from the GenBank database. PCR primers were designed toamplify a conserved region from this sequence. Each primer was 20bases long, and the 5` end of the forward primer (F) and reverseprimer (R) starts from base position 33 and 865, respectively. APCR master mixture for 23 reactions to analyse different birdsamples wasprepared.                           Â
- Fill in the necessary information for the master mix in thefollowing table
No of reactions tomake up | 23 | |||
Vol of each Reaction(µL) | 24 µL +1 µL DNA | |||
Total VolumeRequired | _____µL total | |||
Component | Suppliedas | Final ConcinPCR | µLrequired 1rxn       23 MgCl2 rxn’s | |
MgCl2 | 25 mM | 2.5 mM | ||
Polymerase buffer | 5x | 1x | ||
Primer forward(F) | 10 µM | 1 µM | ||
Primer Reverse(R) | 10 µM | 1 µM | ||
Taq DNApolymerase | 1Units/µL | 1 U per rxn | ||
dNTPs | 25 mM | 1 mM | ||
Template DNA addedlater | - | - | ||
Water | - | - | ||
Total volume | 25 µL |
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