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One can take a fragment of genomic DNA carrying a eukaryotic gene and clone it into...

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Biology

One can take a fragment of genomic DNA carrying a eukaryoticgene and clone it into a bacterial plasmid, containing an origin ofreplication and a selectable marker gene. Although the eukaryoticDNA can be stably replicated in the bacteria, the gene is often notexpressed. Explain four possible reasons why this is the case.

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4.3 Ratings (927 Votes)
1 May be gene is not correctly oriented in the plasmid due to ligation in reverse direction So the gene being read in reverse which is a totaly different sequence 2 Bacteria lacks nucleus where    See Answer
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