Of the original “undiluted†sample from Question 1(see below),10L of a 5-to-1 dilution were used to measure proteinconcentration. With the use of a standard curve, this is found tobe 140g. What is the specific activity of the original sample?Question 1 referenced above: Assayed for LDH activity were 5L of asample that was diluted 6 to 1. The activity in the reactionvessel, which has a volume of 3 mL, is 0.30 U. What is the A/minobserved? What is the relative activity of the original sample? (Icalculated a relative activity of 360 U/mL from question 1 with anA/min change of 0.622) Using this information I get the following:140ug/uL=14ug/uL=14mg/mL x 5 dilutions= 70mg/mL For specificactivity= (U/mL)/(mg/mL)=(360 U/mL)/(70mg/mL)= 5.14 U/mg Does thislook correct?
