Objective 1: You run a transcriptome analysis and find that Lis1 is a gene that is has...

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Medical Sciences

Objective 1:
You run a transcriptome analysis and find that Lis1 isa gene that is has a loss-of-function mutation more often in ASDpatients compared to non-ASD controls. You would like to understandhow this mutation manifests in a rodent model.
Please describe what â€˜loss-of-functionâ€™means.
Â Â Â Â Â Â Â Â Â Â Â Â Â Loss-of-function means that this Lis1 geneâ€™s product(protein) will have less to no function at all. With less function,the gene product will only be partially inactivated, but withcomplete loss of function it is amorphic.
**Please describe how you can examine the effects ofdecreased Lis1 function in mice at the RNA and proteinlevels.
Â Â Â Â Â Â Â Â Â Â Â Â Â**
I need help with the 2nd portion of this question. She isbasically asking how you would measure changes in RNA and changesin protein, but I am not sure what the best method would be to doeither one of these. Qpcr?

Answer & Explanation Solved by verified expert

3.9 Ratings (690 Votes)

To examine the effects of decreased lis1 function in mice at RNA level we can use the following techniques 1 Northern Blotting It shows the size and sequences of the used mRNA molecules A sample of RNA is See Answer

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