o carry out 'blue-white screening', the E.coli plasmid pUC18 carries: A. The N-terminal portion of the lacI gene B. The...

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Biology

o carry out 'blue-white screening', the E.coli plasmidpUC18 carries:
A.
The N-terminal portion of the lacI gene
B.
The N-terminal portion of the lacZ gene
C.
The C-terminal portion of the lacI gene
D.
The C-terminal portion of the lacZ gene
E.
The entire lacI gene

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Blue white screening is based on the activity of betagalactosidase coded by lacZ which cleaves lactose into glucose and galactose The multiple cloning site is present in lacZ sequence When the foreign gene will See Answer

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	A.	The N-terminal portion of the lacI gene
	B.	The N-terminal portion of the lacZ gene
	C.	The C-terminal portion of the lacI gene
	D.	The C-terminal portion of the lacZ gene
	E.	The entire lacI gene