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No GFP 100 25 10 4 2 Counts Counts Counts Counts 50 100 0 10...

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Biology

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No GFP 100 25 10 4 2 Counts Counts Counts Counts 50 100 0 10 Counts 50 0 10 100 50 100 0 10 10 50 10 100 10 0 10 10 50 0 1 10 GFP 87 77 10 GFP 10 10 GFP 82 83 10 104 10 104 81 21 104 10 10 GFP 73 13 104 0 10 10 10 10 104 GFP Figure S11 Transfection Efficiency of the PLAT E Cells and pMX System We transfected the GFP expressing retroviral vector and the control vector at ratios of 1 0 1 3 1 9 and 1 23 into MEFs with a constant amount of total DNA Forty eight hours later cells were photographed under a fluorescence microscope and analyzed by flow cytometry Bars indicate 200 m The analysis showed that 88 83 81 and 73 of transfected cells expressed GFP the mean GFP fluorescence being weaker with decreased concentration of the GFP expressing vector This indicates that 47 0 83 12 0 81 and 0 05 0 7324 of cells transfected with the four 10 or 24 factors expressed all the factors respectively In a 100 mm dish we plated 8 x 105 cells of which 4 x 10 cells should express all the four selected

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