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Ni-NTA resin was used to purify His6-tagged fusion protein Y. What method is used to desorb...

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Biology

Ni-NTA resin was used to purify His6-tagged fusion protein Y.What method is used to desorb the fusion protein Y attached to theNi-NTA resin?

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Recombinant proteins which have histidine tags can be purified using immobilized metal ion chromatography IMAC The Histag can be placed on either the N or Cterminus Optimal binding and therefore purification efficiency is achieved when the Histag is freely accessible to metal ion support Histidine tags have strong affinity for metal ions eg Co2 Ni2 Cu2 and Zn2 One of the first support materials used immobilized iminodiacetic acid which can bind metal ions and allow    See Answer
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