Mr. Jones’ ninth grade class is studying the influence oftemperature on respiration rate in goldfish. Each of his 24students has a single goldfish isolated in a goldfish bowl halffull of de-chlorinated tap water at 15°C. Each student is allowedto add a random amount of either chilled tap water (5°C) or warmedtap water (30°C) very slowly for 5 minutes to gently adjust thetemperature of the water in the bowl. The goldfish are allowed anadditional 5 minutes of acclimation time after the temperature inthe bowl has equilibrated. Then each student records thetemperature of the water to the nearest 0.1°C using a digitalthermometer and the goldfish respiration rate (the number of timesthe operculum or gill cover opens) during a 60 second period. Theclass hypothesis (H₁) is that respiration rate (cycles /min) will increase with increasing temperature (°C).

The independent variable is temperature and is continuous. Thedependent variable is respiration rate and is continuous. Theobservations are paired in the sense that each temperature has onlyone respiration rate. However, there is a clear expectation fromthe class hypothesis that the independent variable is causing thechange in the dependent variable.

Student	Temperature (C)	Respiration rate (cycles/min)
1	15.0	20
2	13.5	18
3	17.9	25
4	24.3	36
5	18.2	28
6	12.4	17
7	11.9	16
8	14.3	19
9	16.5	23
10	13.2	18
11	15.9	21
12	21.3	32
13	22.7	34
14	12.2	16
15	10.9	15
16	25.2	40
17	6.3	11
18	9.3	14
19	15.1	20
20	13.4	18
21	5.1	10
22	8.3	12
23	9.2	13
24	11.4	15

1. Which of the following is the test statistic (observed) forthis experiment?

A. SE= -1.564

B. F= 600.36

C. df=22

D. intercept= -1.114

2. Using the relationship you measured between temperature andrespiration rate, calculate the expected temperature whererespiration rate would equal 0

A. 0.739

B. 1.5072

C. 24

D. 1.84 x 10^-17