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  3. mol per g wet wt caecal contents 120 100 80 60 40

mol per g wet wt caecal contents 120 100 80 60 40 20 0 H...

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mol per g wet wt caecal contents 120 100 80 60 40 20 0 H Acetate Obese oblob Lean ob 3 5 b Butyrate b Propionate 4 01 3 0 2 5 2 0 Lean Obese C Increase in body fat 60 40 20 0 Donor oblob Figure 3 Biochemical analysis and microbiota transplantation experiments confirm that the ob ob microbiome has an increased capacity for dietary energy harvest a Gas chromatography mass spectrometry quantification of short chain fatty acids in the caeca of lean n 4 and obese n 5 conventionally raised C57BL 6J mice b Bomb calorimetry of the faecal gross energy content kcal g of lean ob n 9 and obese ob ob n 13 conventionally raised C57BL 6J mice c Colonization of germ free wild type C57BL 6J mice with a caecal microbiota harvested from obese donors ob ob n 9 recipients results in a significantly greater percentage increase in total body fat than colonization with a microbiota from lean donors n 10 recipients Total body fat content was measured before and after a two week colonization using dual energy X ray absorptiometry Mean values s e m are plotted Asterisks indicate significant differences two tailed Student s t test of all datapoints P 0 05 P 0 01 p 0 001

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