Logo
Home Study Material Contact Search
AI Chat FREE
  1. sciences
  2. chemistry
  3. materials: reaction buffer 0.5 m tris hcl ph 8.0 w

Materials: Reaction Buffer 0.5 M Tris HCl pH 8.0 with 5 mM MgCl2 Alkaline phosphatase: 500 μg/mL...

60.1K

Verified Solution

Question

Chemistry

Materials:

Reaction Buffer 0.5 M Tris HCl pH 8.0 with 5 mM MgCl2

Alkaline phosphatase: 500 μg/mL stock solution in the bufferabove. Keep enzyme on ice!

p-nitrophenyl phosphate: 1000 μM and 10000 μM stock solutions inreaction buffer

1) Using the enzyme stock solution above and the dilutionequation, calculate the volumes needed to prepare 2 mL ofthree enzyme dilutions consisting of 100 μg/mL, 125 μg/mL,and 150 μg/mL. Use the reaction buffer to makethese dilutions.

2) Calculate the amount of substrate needed foreach of the following concentrations 10 μM, 25 μM, 50 μM, 100 μM,200 μM, 500 μM, 1000 μM, and 2000 μM using the two stock solutionconcentrations listed above. Use the 1000 μM stock solution tocalculate dilutions from 10-200 μM and the 10,000 μM stock solutionto calculate from 500 μM-2000 μM. The final volume is 1.0 mL (or1000 μL) for each dilution. Also calculate the volume ofbuffer needed, given that we will add 100 μL of the enzymesolution and the final volume is 1.0 mL (or 1000 μL) for eachdilution.

Answer & Explanation Solved by verified expert
3.7 Ratings (640 Votes)
    See Answer
Get Answers to Unlimited Questions

Join us to gain access to millions of questions and expert answers. Enjoy exclusive benefits tailored just for you!

Membership Benefits:
  • Unlimited Question Access with detailed Answers
  • Zin AI - 3 Million Words
  • 10 Dall-E 3 Images
  • 20 Plot Generations
  • Conversation with Dialogue Memory
  • No Ads, Ever!
  • Access to Our Best AI Platform: Flex AI - Your personal assistant for all your inquiries!
Become a Member

Other questions asked by students

Finance
1.2K views

2. Please answer with care and attention to all questions. Explain as much as you can...

Chemistry
1.2K views

Calculate the enthalpy of the reaction 4B(s)+3O2(g)→2B2O3(s) given the following pertinent information: B2O3(s)+3H2O(g)→3O2(g)+B2H6(g), ΔH∘A=+2035 kJ 2B(s)+3H2(g)→B2H6(g), ΔH∘B=+36 kJ...

Physics
1.2K views

A charge particle is revolving around a central charge with frequency 2 x 1015 Hz...

Calculus
1.2K views

Find the area of the region bounded by 72 cos 20

Algebra
1.2K views

Find the value of x in the next 3 diagrams to nearest tenth 10 2...

Algebra
1.2K views

Use the table below to fill in the missing values 0 1 234567 8 9...

Accounting
1.2K views

Racer Industries is currently purchasing Part No.76 from an outside supplier for $90 per unit....

Accounting
1.2K views

In addition, during the year, the company completed the disposal of its plastics business and...

Accounting
1.2K views

Taxpayers who have more than one _________ may find at the end of the year...

Accounting
1.2K views

Condensed Consolidated Balance Sheets - USD ($) $ in Thousands Dec. 31, 2016 Dec. 31,...

Accounting
1.2K views

I want to answer in less than an hour and get a high rating ...

Accounting
1.2K views

Data table Requirement 1. If monthly sales are 1,200 tech shirts, what is the full...