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Mary performs a PCR reaction and runs it on an agarose gel, using 100-bp ladder as...

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Biology

Mary performs a PCR reaction and runs it on anagarose gel, using 100-bp ladder as her molecular weight standard.She images the gel using UV light and sees NObands (no band in her PCR reaction and no bands for her ladder).Which of the following could explain these results?Explain each answer.

1. Mary forgot to add Gel Red when pouring the gel.

2. Mary's PCR product had an unusually high % of G andC bases.

3. Mary connected the electrodes to her gelincorrectly, swapping the positive and negativepoles.

Answer & Explanation Solved by verified expert
3.8 Ratings (498 Votes)
1GEL RED is an intercalating nucleic acid stain used in molecular biology for agarose gel electrophoresis Gel Red is structurally closely related to ethidium bromide and consist 2 ethidium subunits that are bridge by a linear spacer when exposed to UV light it will floresce with    See Answer
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