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Make a comparative chart on the main methods of protein quantification. Method Reagents Equipment characteristics Bradford Biuret Lowry BCA (bicinconinic acid)

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Question

Biology

Make a comparative chart on the main methods of proteinquantification.

Method
Reagents
Equipment
characteristics
Bradford
Biuret
Lowry

BCA (bicinconinic acid)

Answer & Explanation Solved by verified expert
3.7 Ratings (405 Votes)
Method Regents Equipments Characteristics Bradford Coomassie Brilliant Blur G250 Preparation Dissolve 100mg of G250 in 50ml 95 ethanol 100ml 85 phosphoric acid After complete dissolution dilute it to 1L with water and then filter Light spectrophotometer that can detect 595nm    See Answer
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