Make 25 mL of a single solution containing 10 mM MOPS [MW=209 g/mole]. How many grams...

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Biology

Make 25 mL of a single solution containing 10 mM MOPS [MW=209g/mole]. How many grams MOPS do you need?

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Ans. Given,

Â Â Â Â Â Â Â Â Â Â Â Volume of solution = 25.0 mL = 0.025 LÂ Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â ; [1 mL = 0.001 L]

Â Â Â Â Â Â Â Â Â Â Â Molarity of solution = 10.0 mM = 0.010 MÂ Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â ; [1 mM = 0.001 M]

Now,

Â Â Â Â Â Â Â Â Â Â Â Required moles of MOPS = Molarity x Volume of solution in liters

Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â = 0.010 M x 0.025 L

Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â = (0.010 mol/ L) x 0.025 LÂ Â Â Â Â Â Â Â Â Â Â Â Â Â ; [1 M = 1 mol/ L]

Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â = 0.00025 mol

Required mass of MOPS = Required moles x Molar mass

Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â = 0.00025 mol x (209.0 g/ mol)

Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â = 0.05225 g

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