Lactate dehydrogenase catalyzes the conversion of pyruvate into lactate. kinetics experiment was done to test the effect...

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Biology

Lactate dehydrogenase catalyzes the conversion of pyruvateinto lactate. kinetics
experiment was done to test the effect of the inhibitoroxamate on the ability of
lactate dehydrogenase to convert pyruvate into lactate. Thedata with and without
inhibitor was plotted as a Lineweaver-Burk plot. The velocityis in units of umol/min
and the concentration of pyruvate is in units of MM
The equation of the line of best fit for the reaction withoutoxamate is: y 3.1x +7.4
The equation of the line of best fit for the reaction withoxamate is: y = 5.4x+29
What are the values of KM. Vmax, KMaPP and Vmax aPP? What typeof inhibitor is oxamate?
Please show all your calculations. Round your final answers tothe nearest 12th and include
the correct units.

that the entire question

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3.6 Ratings (342 Votes)

In an LB plot we have 1S on the Xaxis and 1V on theyaxis We the resultant is a straight line represented bywhere y is the y coordinate of a given point m is the See Answer

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