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Lab Practical I – Microscopy, Aseptic technique, Bacterial culturing/selection, and Staining: Briefly describe the most salient points...

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Medical Sciences

Lab Practical I – Microscopy, Aseptic technique,Bacterial culturing/selection, and Staining:

Briefly describe the most salient points for eachsection. Remember, if you know why the

assay is performed, how it works, and how to interpretit you will do very well on the final.

Section 3-1 and 3-5: Microscopy, Simple stains, andSmears

Section 1-3 and 1-4: Streaking plates, Transfer, andColony Selection

Section 3-7: Gram Staining

Section 3-12, 3-13, and 5-28: Motility (Microscope-wetmount), Flagella Staining, and Motility

test

Section 3-8: Acid Fast Staining

Section 3-9, and 3-10: Capsule and EndosporeStaining

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4.4 Ratings (898 Votes)
lab practical I Microscopy Microbiology microscopes are used to identify and visualize some tricky samples including bacteria algae and fungi Power objective lenses with high resolution as well as observation techniques such as phase contrast microscopy and darkfield microscopy are key for observing some of earths smallest creatures Dark field microscopy done to see Treponema Pallidium The following types of microscopes are in use now Brightfield or light microscope Dark field microscope Phase contrast microscope Fluorescence microscope Electron microscope Aseptic technique Aseptic technique is a standard healthcare practice that helps prevent the transfer of germs to or from an open wound and other susceptible areas on a patients body Aseptic technique helps prevent healthcareassociated infections HCAIs An HCAI is an infection that a person acquires as a result of treatment from a healthcare professional Common HCAIs include catheterassociated urinary tract infection central lineassociated bloodstream infection Clostridium difficile infection surgical site infection ventilatorassociated pneumonia there are several different aspects of aseptic technique practices barriers Barriers Barriers prevent the transfer of germs between healthcare professionals patients and the environment Aseptic barriers include sterile gloves sterile gowns sterile masks sterile drapes protective wrappers on sterilized instruments patient and equipment preparation disinfecting a patients skin using antiseptic wipes sterilizing equipment and instruments before a procedure keeping sterilized instruments inside plastic wrappers to prevent contamination before use environmental controls Maintaining an aseptic field involves keeping doors closed minimizing movement in and out of the aseptic field limiting entry to necessary personnel only permitting only one patient per aseptic field contact guidelines Bacterial Culturing Culture media are required to isolate die bacteria from the    See Answer
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